High-Resolution Melting Analysis Using the Roche LightCycler Instrument for Rapid and Reliable Methylation Detection in Ar

PENZBERG, Germany--(BUSINESS WIRE)-- Promoter hypermethylation is a frequent mechanism for the repression of gene transcription in cancer and is regarded as one of the hallmarks of cancer. Analysis of DNA methylation is a promising tool for early cancer detection, risk assessment, and response to therapy. In a recent study (1), quantitative high-resolution melting analysis (HRM) with the Roche (Pink Sheets: RHHBY) (SWX:RO) (SWX:ROG) LightCycler Instrument was used for promoter methylation for analysis of formalin-fixed paraffin-embedded (FFPE) tissues. Since FFPE tissue samples are the largest source of material from normal controls and diseased tissues, their use is of inestimable value for research. Methodical evaluations are of high importance for demonstrating robustness and sensitivity of the assay, thus facilitating its establishment as a research tool and possibly a future routine test.

The aim of the study was to establish and evaluate HRM assays for detection of promoter methylation on archival FFPE tissues from individuals with colorectal cancer. As proof of the principle, the researchers demonstrated the applicability of HRM for detection of promoter methylation using assays for O6-methylguanine-DNA methyltransferase (MGMT), adenomatous polyposis coli (APC), glutathione S-transferase P1 (GSTP1), and phosphatase and tension homolog deleted on chromosome 10 (PTEN) promoters in methylated DNA dilution matrix. In a second step, HRM assays for MGMT and APC were tested on DNA isolated from fresh and FFPE human cancer cell lines. These established MGMT and APC HRM assays were analyzed using archival FFPE colorectal tumor specimens. Methylated DNA levels as low as 1% were reproducibly detected in a background of unmethylated DNA. For certain applications, such as detection of rare events or risk stratification of individuals based on methylation status of specific markers, high sensitivity of the assay is important. This sensitive assay can be adapted and used to detect low amounts of methylated cells within a tumor, or even to detect low numbers of tumor cells in the background of non-tumor cells in lymph nodes and other organs.

The most popular approaches for DNA methylation detection rely on the treatment of genomic DNA with sodium bisulfite, which converts cytosine into uracil while leaving 5-methyl cytosine unmodified. This modification results in a sequence difference allowing for identification of methyl cytosines in a subsequent PCR amplification. The most precise methylation profiling can be achieved by bisulfite sequencing, which allows identification of single methyl cytosines. Several simpler PCR-based methods, which are especially important for small scale research labs, have also been developed. One of these newer and easier methods is high-resolution melting analysis (HRM), based on the “melting” properties of DNA in solution. The principle of this method is that bisulfite-treated DNA templates with different contents of methyl cytosine can be distinguished by melting analysis based on differences in melting temperatures. HRM is a relatively simple and cost-effective method, since it does not require expensive probes and reference gene assays for normalization. With HRM, all CpGs within the amplicon are analyzed, enabling the assay to distinguish heterogeneous from homogeneous methylation by the shape of the melting curve. This ability can be of importance, because methylation patterns at promoter CpG islands are typically not homogeneous.

(1) Roche Applied Science Cancer Research Application Note No. 3: M. Malic, M. Pichler, E. Heitzer, J. Strutz, N. Dandachi (2009) High quality assessment of DNA methylation in archival tissues from patients with colorectal cancer using quantitative high-resolution melting analysis, www.cancer-research.roche.com.

About Roche
Headquartered in Basel, Switzerland, Roche is a leader in research-focused healthcare with combined strengths in pharmaceuticals and diagnostics. Roche is the world’s largest biotech company with truly differentiated medicines in oncology, virology, inflammation, metabolism and CNS. Roche is also the world leader in in-vitro diagnostics and tissue-based cancer diagnostics, and is a pioneer in diabetes management. Roche’s personalized healthcare strategy aims at providing medicines and diagnostic tools that enable tangible improvements in the health, quality of life and survival of patients. In 2009, Roche had over 80,000 employees worldwide and invested almost 10 billion Swiss francs in R&D. The Group posted sales of 49.1 billion Swiss francs. Genentech, United States, is a wholly owned member of the Roche Group. Roche has a majority stake in Chugai Pharmaceutical, Japan. For more information: www.roche.com.

For life science research only. Not for use in diagnostic procedures.

All trademarks used or mentioned in this release are protected by law.

LIGHTCYCLER is a trademark of Roche.



CONTACT:

Roche Diagnostics
Dr. Burkhard Ziebolz
Phone: +49 8856 604830
Email: [email protected]

KEYWORDS:   United States  Europe  North America  Germany  Switzerland

INDUSTRY KEYWORDS:   Stem Cells  Health  Genetics  Hospitals  Medical Devices  Oncology  Pharmaceutical  Research  FDA  Medical Supplies  Science

MEDIA:

Logo
 Logo

Suggested Articles

The eight-year deal will initially cover lupus drug Benlysta and could expand to other GSK specialty-care products in the future.

Amarin had big plans for Vascepa after a big label expansion last year, but it lost a patent fight—and now a generic has won FDA approval.

Intercept Pharmaceuticals, eager to market its potential nonalcoholic steatohepatitis medicine obeticholic acid, will have to keep waiting.